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In the discussion above, we emphasize that increases in the discriminating power of selection can favor streamlining, leaving open the question of how small genome size confers a fitness advantage. In streamlined marine organisms there is a trend to higher proportions of nucleotides (A+T) and amino acids (for example, the substitution of lysine for arginine) that have lower N content, indicating that streamlining selection acts to reduce the amount of nitrogen needed for cell replication ( Blazer Mid Trainers In Black 371761033 Black Nike Looking For Cheap Online CY2M8b
; Grzymski and Dussaq, 2012 ). Evidence that streamlining selection is driven by fitness advantages of reduced cellular P quotas is, so far, not convincing, but that may be because the data needed to test this hypothesis are less readily available ( Cheap Big Sale Bulk Designs hi top sneakers Brown Rick Owens Cheap Shopping Online Discount Visit QT44jrhC
); in the oceans, N and P tend to be co-limiting ( Falkowski et al., 1998 ). Comparative studies of streamlining in P-limited and P-replete systems might shed light on this issue.

Reductions of cellular N and P quotas are not the only factors that have potential to drive streamlining selection. Streamlined marine bacteria include some of the world’s smallest organisms ( Chisholm et al., 1992 ; Rappé et al., 2002 ; FOOTWEAR Sandals Belstaff Inexpensive Cheap Online Clearance Discount Affordable Clearance High Quality Cheap Enjoy kytljE
), raising the possibility that increased surface-to-volume ratios might also drive streamlining in some cases. A metaproteomics study of Pelagibacter in the ultra-oligotrophic Sargasso Sea showed that a strikingly high (67%) proportion of cellular protein is devoted to transport functions, suggesting that high surface-to-volume ratios are a factor in the success of these very small (ca 0.01 μm 3 ), streamlined cells ( Garavani V Rivet suede pumps Valentino With Paypal For Sale XNtYbdc5z
). In a twist on this perspective, Thingstad argued that bacterioplankton may increase their cell size to succeed by defense specialism– –growing too large for their predators. He theorized that the surface-to-volume ratio is less important than the surface-to-‘cell requirements of a limiting element’, and that larger cells can maintain the benefits of smaller cells by scaling up their cell composition by accumulating non-limiting nutrients such as carbon in an N- and P-limited system ( Thingstad et al., 2005 ). The theory that selection favors small genomes because they can be replicated more rapidly, thus shortening generation times, has largely been ruled out by studies that found no correlation between generation time and genome size ( Mira et al., 2001 ; Lynch, 2006 ; Touchon and Rocha, 2007 ; Vieira-Silva and Rocha, 2010 ).

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I've been using the services at The Monogram shop for 15+ years. Sometimes I purchase products they offer in their retail shop, but most times I find items that I want to personalize and bring them to their store for monogramming. (Most stores won't let you bring in your "from home" or store bought stuff to be monogrammed... they require you to purchase from their inventory.) They've monogrammed my gifts for graduations, newborns, Christmas, birthdays, teacher gifts, etc... They have a limited selection of styles and that can be disappointing because there are some very chic elaborate monograms that I see on-line that are very inspiring. But the flip-side is that their monograms although basic are affordable. I've occasionally forgotten that their hours are limited and found myself pulling up to the store and being frustrated because I forgot their hours of operation... but that's my fault. They offer a very basic service with reliable quality and if they don't think they can fulfill your request they let you know. (this has happened to me a few times.... they know what their equipment can accommodate.) I know that they can personalize things for sports teams, businesses and more. I've been pleased with the service and product I've received. Plus it's unique to have a mother/daughter locally owned business thrive in this world of on-line retail. I've enjoyed watching the grandchildren/children grow up from being newborns napping in the back room, to becoming rambunctious kiddos running happily through the showroom.

pretty much has the most inconvient hours ever. they close at 5, not open on weekends, and more than once I've gone there during the day to find them closed for vacation or such. I get it's a family owned business, but are there no other associates to take walk in sales or for order pick ups? I love monogramming things and they have a great selection for in store purchases, but the location and terrible hours lead me to go somewhere else(usually online). I wish they were in the Barracks area or even the new stonefield. The work the shop does for items I've brought in is great, but can't get past the bad location and hours.

1 star because I can't give 0... First bad encounter - selected a trick-or-treat bag for my daughter wanted it monogramed. When I picked it up, the name wasn't even centered on the bag! It looked bad to me, but I needed the bag that day, so I took it as it was. Second bad encounter - given the already limited hours (see other review) they should make every effort to be open during those hours. I carved hours out of my day to go at lunch time and ... they were closed! Just a "be back @" sign in the window. Third bad encounter - after viewing their website for ideas, I selected an item and called to order it. When I went to pick it up, the item was significantly more expensive. When I said that I was a bit surprised at the difference, the woman said, "Oh, the website isn't supposed to be accessible right now. Most of the prices are wrong," I mentioned that the correct answer to my inquiry was, "I'm sorry for the confusion." I then got the same snippy response about the website needing to be offline and a extremely insincere appology. I always try to shop locally, but I will be taking all my future needs online. After issues one and two above, I did order another treat bag (for Easter) online. It was done quickly, correctly, and without any of the issues I seem to have whenever I visit The Monogram Shop.

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